RNA-seq of Larix decidua: Inoculated at 3 days - SRA

SRX14259928: RNA-seq of Larix decidua: Inoculated at 3 days
1 ILLUMINA (Illumina NovaSeq 6000) run: 5.7M spots, 1.7G bases, 555.7Mb downloads

Design: RNA samples were sent to the Oxford Genomics Centre (Oxford, United Kingdom) for library preparation and sequencing. Polyadenylated transcript enrichment was performed with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), and then individual libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs). Individual libraries were normalised and the pooled library was paired-end-sequenced was performed on the Illumina NovaSeq6000 with a read length of 150 bp.

Submitted by: University of Oxford

Study: Comparative transcriptomic responses of European and Japanese larches to infection by Phytophthora ramorum

PRJNA809546 • SRP361052 • All experiments • All runs

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Phytophthora ramorum severely affects both European larch (EL) and Japanese larch (JL) trees as indicated by high levels of mortality. Field observations show that EL is less severely affected suggest it is less susceptible to P. ramorum than JL; however, controlled inoculations have produced inconsistent or non-statistically significant results. The present study aimed to compare RNA transcript accumulation profiles in EL and JL in response to inoculation with P. ramorum to improve our understanding of their defence responses.

Sample:

SAMN26186935 • SRS12078947 • All experiments • All runs

Organism: Larix decidua

Library:

Name: WTCHG_758577_73315307

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Runs: 1 run, 5.7M spots, 1.7G bases, 555.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR18109123	5,716,457	1.7G	555.7Mb	2022-08-01

ID:: 20206643

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